Conformational lability of poly(dG-m5dC):poly(dG-m5dC)

Conformational lability of herpesvirus protein VP22.

The herpesvirus protein VP22 traffics between cells, being exported from expressing cells in a non-Golgi-dependent manner and localizing in the nuclei of surrounding cells. This transport is retained in certain VP22 fusion proteins, making VP22 a candidate for use in macromolecular drug delivery. In an effort to understand the physical basis for this activity, we have initiated structural studi...

Title Conformational Lability of Herpes Virus Protein VP 22

The Lability Test.

Methods The test is a combined one. One hand is put into ice-cold water and at the same time constriction is applied to the neck in order to increase the venous pressure in the head. The latter is done by means of an ordinary sphygmo-manometer with the blood pressure cuff round the neck. We have only used a pressure of 40 mm. Hg. in the cuff. Bloomfield (1947) and Bloomfield and Kelterman (1947...

Conformational lability in the class II MHC 310 helix and adjacent extended strand dictate HLA-DM susceptibility and peptide exchange.

HLA-DM is required for efficient peptide exchange on class II MHC molecules, but its mechanism of action is controversial. We trapped an intermediate state of class II MHC HLA-DR1 by substitution of αF54, resulting in a protein with increased HLA-DM binding affinity, weakened MHC-peptide hydrogen bonding as measured by hydrogen-deuterium exchange mass spectrometry, and increased susceptibility ...

Bronchial lability in cystic fibrosis.

to the severity of pulmonary involvement assessed clinically and radiologically, and with those found in asthmatic children and those with a history of wheezy bronchitis in early childhood. In patients with CF, bronchial lability was found even in those with minimal pulmonary involvement. The lability was due not only to bronchoconstriction but to a dilatation which was greater than occurs in n...